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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha : |
16/11/2015 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MEDEROS, A.; KELTON, D.; PEREGRINE, A.S.; VANLEEUWEN, J.; FERNÁNDEZ, S.; LEBOEUF, A.; MENZIES, P.; MARTIN, R. |
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Afiliación : |
AMERICA ESTHER MEDEROS SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Evaluation of the utility of subjective clinical parameters for estimating fecal egg counts and packed cell volume in Canadian sheep flocks. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Veterinary Parasitology, 2014, v.205, p. 568?574. |
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ISSN : |
0304-4017 |
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DOI : |
10.1016/j.vetpar.2014.08.030 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 February 2014 / Received in revised form 8 August 2014 / Accepted 30 August 2014. |
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Contenido : |
ABSTRACT.
A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that
may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring)
and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithmetic mean EPG was 907 and 237 for lambs, and 458 and 246 for ewes, in ON and QC, respectively. Results from simple correlations indicated that PCV was negatively correlated with lnEPG (r = −0.255; r2 = 6.5%) and FAMACHA (r = −0.312; r2 = 9.7%), and positively correlated with BCS (r = 0.317; r2 = 10%). LnEPG was negatively correlated with BCS (r = −0.232; r2 = 5.4%) and PCV (r = −0.255; r2 = 6.5%), but positively correlated with FAMACHA (r = 0.178; r2 = 3.2%) and DS (r = 0.086; r2 = 0.7%). Results from the models indicated that PCV and lnEPG residuals were negatively correlated with FAMACHA, FCS and almost all categories of BCS and DS, although the correlations were very low. The main results from this study suggested that none of the subjective clinical parameters evaluated were highly correlated with PCV or lnEPG and therefore were not good predictors of lnEPG or PCV on the studied farms in Ontario and Quebec.
© 2014 Elsevier B.V. All rights reserved MenosABSTRACT.
A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that
may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring)
and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithm... Presentar Todo |
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Palabras claves : |
NEMATODOS GASTROINTESTINALES. |
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Thesagro : |
OVINOS. |
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Asunto categoría : |
--
L73 Enfermedades de los animales |
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Marc : |
LEADER 03399naa a2200265 a 4500
001 1053920
005 2019-10-15
008 2014 bl uuuu u00u1 u #d
022 $a0304-4017
024 7 $a10.1016/j.vetpar.2014.08.030$2DOI
100 1 $aMEDEROS, A.
245 $aEvaluation of the utility of subjective clinical parameters for estimating fecal egg counts and packed cell volume in Canadian sheep flocks.$h[electronic resource]
260 $c2014
500 $aArticle history: Received 12 February 2014 / Received in revised form 8 August 2014 / Accepted 30 August 2014.
520 $aABSTRACT. A study was conducted in sheep on Canadian farms to describe the relationship between packed cell volume (PCV) or fecal egg counts (FEC) and subjective clinical parameters that may indicate the severity of parasitic gastroenteritis. Twenty-one farms in Ontario (ON) and 8 farms in Quebec (QC) were purposively selected and visited during April?May (spring) and August (summer) 2007. At each farm visit, blood and fecal samples were collected from 10 ewes and 10 female lambs; body condition score (BCS), dag score (DS), fecal consistency score (FCS) and FAMACHA score were recorded for all sampled sheep. Packed cell volume was determined for all blood samples, and FEC were performed for all fecal samples. Summary statistics and simple correlations were performed for the parameters recorded. Two mixed models with random effects at the farm level were developed; one using PCV as the response variable and another using the natural log of eggs per gram of feces (lnEPG). Finally, the residuals from both models were correlated to the covariates in the models. The mean PCV values during the spring were 29.7% and 36.7% for lambs, and 28.8% and 31.1% for ewes, in ON and QC, respectively. During the summer, the mean PCV was 32.0% and 32.8% for lambs, and 30.1% and 29.9% for ewes, in ON and QC, respectively. The arithmetic mean FEC per gram of feces (EPG) during the spring was 3 and 2 for lambs, and 1266 and 789 for ewes, in ON and QC, respectively, whereas during summer the arithmetic mean EPG was 907 and 237 for lambs, and 458 and 246 for ewes, in ON and QC, respectively. Results from simple correlations indicated that PCV was negatively correlated with lnEPG (r = −0.255; r2 = 6.5%) and FAMACHA (r = −0.312; r2 = 9.7%), and positively correlated with BCS (r = 0.317; r2 = 10%). LnEPG was negatively correlated with BCS (r = −0.232; r2 = 5.4%) and PCV (r = −0.255; r2 = 6.5%), but positively correlated with FAMACHA (r = 0.178; r2 = 3.2%) and DS (r = 0.086; r2 = 0.7%). Results from the models indicated that PCV and lnEPG residuals were negatively correlated with FAMACHA, FCS and almost all categories of BCS and DS, although the correlations were very low. The main results from this study suggested that none of the subjective clinical parameters evaluated were highly correlated with PCV or lnEPG and therefore were not good predictors of lnEPG or PCV on the studied farms in Ontario and Quebec. © 2014 Elsevier B.V. All rights reserved
650 $aOVINOS
653 $aNEMATODOS GASTROINTESTINALES
700 1 $aKELTON, D.
700 1 $aPEREGRINE, A.S.
700 1 $aVANLEEUWEN, J.
700 1 $aFERNÁNDEZ, S.
700 1 $aLEBOEUF, A.
700 1 $aMENZIES, P.
700 1 $aMARTIN, R.
773 $tVeterinary Parasitology, 2014$gv.205, p. 568?574.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
15/08/2022 |
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Actualizado : |
01/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
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Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
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Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
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DOI : |
10.1002/mrd.23638 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
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Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
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Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03782naa a2200409 a 4500
001 1063525
005 2022-12-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1002/mrd.23638$2DOI
100 1 $aPASSOS, J.R.S.
245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.
520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.
653 $aFOLLICLE
653 $aOVARY
653 $aOVINE
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aPROTEINS
653 $aREPRODUCTION
700 1 $aGUERREIRO, D.D.
700 1 $aKAMILA S. OTÁVIO
700 1 $aDOS SANTOS-NETO, P.C.
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ?OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aVASCONCELOS, F.R.
700 1 $aBEZERRA, M.J.B.
700 1 $aSILVA, R.F.
700 1 $aLIMA, L.F.
700 1 $aFIGUEIREDO, J.R.
700 1 $aBUSTAMANTE-FILHO, I.C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A.A.
773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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